V.G. Shivdare College of Arts, Commerce and Science, Solapur

SCIENCE

CERTIFICATE COURSE

MOLECULAR DIAGNOSTICS

Course Profile

Year of Establishment : 2021-22

Course Duration: 6 Months

Eligibility for Admissions: Any Life Sciences Graduate / Post Graduate, Any Medical Graduate / Post Graduate

Course fees per Student: - Rs.6000 /-

Student Intake for course: 25

Job opportunities:

Laboratory Assistant in Molecular Biology / Diagnostics Laboratories

Young Professional (Assisting scientist in-charge in Molecular Biology / Genomics / genome sequencing)

Medium of Instruction – English

ANY SCIENCE COLLEGE PROVIDED WITH MOLECULAR BIOLOGY LABORATORY CAN APPLY FOR THE AFFILIATION OF THIS COURSE.

Essential Requirement:

1.  Lecture hall with all essential facilities.

2.  Biochemical OR Microbiology Laboratory having equipments required to do molecular biology work  OR

3.  Molecular Biology Laboratory with all essential facilities

Staff Qualifications:

M. Sc. Biotechnology/Biochemistry/Microbiology, M.Sc. or Ph.D. in Molecular Biology and Genetics

Non – teaching Staff (Lab. Assistant): B.Sc. Biotechnology/Biochemistry/Microbiology/ D.Pharm.

Course Evaluation

Theory                                                 100 Marks

Practical                                              100 Marks

(80 Marks University Assessment and 20 College/Internal Assessment Marks)

Objectives:

1. To prepare skilled manpower needed in Molecular Diagnostics laboratory.

2. To carry out Molecular Biology work in Departments of medical and sciences colleges.

3. To inculcate in candidates concepts of Molecular Biology research for getting opportunities in Research Institutes working in the similar field.

Fee Structure:

Course fees per Student: - Rs. 6000 /-

 Academic calendar:

I^st batch

II^nd batch

Student Learning Outcomes :

Course Information :

Teaching Staff :

Non-teaching :

Revised syllabus (2022-23)
SYLLABUS

THEORY

UNIT I                                                                                    (15 Periods)

Enzyme Immunoassays:

Comparison of enzymes available for enzyme immunoassays, conjugation of enzymes. Solid phases used in enzyme immunoassays. Homogeneous and heterogeneous enzyme immunoassays. Enzyme immunoassays after immuno blotting. Enzyme immuno histochemical techniques. Use of polyclonal or monoclonal antibodies in enzymes immuno assays. Applications of enzyme immunoassays in diagnostic microbiology

UNIT II                                                                                  (15 Periods)

Molecular methods in clinical microbiology:

Applications of PCR, Molecular markers (RFLP, RAPD, AFLP etc.), Nuclear hybridization methods, Single nucleotide polymorphism and plasmid finger printing in clinical microbiology

Laboratory tests in chemotherapy: Susceptibility tests: Micro-dilution and macro-dilution broth procedures. Susceptibility tests:Diffusion test procedures. Susceptibility tests: Tests for bactericidal activity. Automated procedures for antimicrobial susceptibility tests.

UNIT III                                                                                 (18 Periods)

Automation in microbial diagnosis, rapid diagnostic approach including technical purification and standardization of antigen and specific antibodies. Concepts and methods in idiotypes. Antiidiotypes and molecular mimicry and receptors. Epitope design and applications. Immunodiagnostic tests. Immuno florescence. Radioimmunoassay.

UNIT IV                                                                                 (12 Periods)

GLC, HPLC, Microscopy (Inverted, bright field, fluorescence, Electron microscopy), flow cytometry and cell sorting. DNA sequencing

UNIT V                                                                                  (10 Periods)

Handling of Instruments and Laboratory Management: Safety regulation, Good Lab Practices, Quality control methods and maintenance of laboratory records, First aid, Correct practices for handling and disposal of biological, Queuing & Maintaining of Lab material & Instruments., Lab Standard.
 

PRACTICALS

(Wherever wet lab experiments are not possible the principles and concepts can be demonstrated through any other material or medium including videos/virtual labs etc.)

1.  Introduction to common laboratory instruments colorimeter, Spectrophotometer, centrifuge, pH meter, microscope, autoclave, hot air oven

2.  Perform/demonstrate RFLP and its analysis

3.  Kirby-Bauyer method (disc-diffusion method) to study antibiotic sensitivity of a bacterial culture

4.  A kit-basd detection of a microbial infection (Widal test)

5.  Study of Electron micrographs (any four).

6.  Perform any one immuno diagnostic test (Typhoid, Malaria, Dengue)

7.  Isolation of genomic DNA from pathogenic bacteria

8.  Analysis of PCR results

9.  Record writing and methodology of reporting.

10.  Visit to Molecular Biology laboratory OR 3 month on-the-job training in any recognized Pathology / Microbiology / Molecular testing laboratory (Optional)

SUGGESTED READING

1.  Practical Biochemistry, Principles and Techniques, Keith Wilson and John Walker

2.  Advanced Instrumentation, Data Interpretation, and Control of Biotechnological Processes,

J.F. Van Impe,Kluwer Academic

3.  Ananthanarayan R and Paniker CKJ. (2005). Textbook of Microbiology. 7th edition (edited by

Paniker CKJ). University Press Publication.

4.  Brooks GF, Carroll KC, Butel JS and Morse SA. (2007). Jawetz, Melnick and Adelberg’s Medical Microbiology. 24th edition. McGraw Hill Publication.

5.  Goering R, Dockrell H, Zuckerman M and Wakelin D. (2007). Mims’ Medical Microbiology.

4th edition. Elsevier.

7.  Willey JM, Sherwood LM, and Woolverton CJ. (2008). Prescott, Harley and Klein’s Microbiology. 7th edition. McGraw Hill Higher Education.

8.  Microscopic Techniques in Biotechnology, Michael Hoppert

Activities:

Students enrolled for Certificate Course in Molecular Diagnostics get experience by practicals related to Molecular Biology in laboratory. Students also get practical experience by visiting or/and training at various Pathology, Microbiology, Serology/Immunology and Molecular biology laboratories such as Muley Lab., Kadadi Lab., Potdar Lab. and Diagnostics labs at Ashwini Sahakari Rugnalaya and Research Centre, Solapur.

Achievements

SCIENCE

CERTIFICATE COURSE

MOLECULAR DIAGNOSTICS

Felicitation by Chandrakant Dada Patil of Sayli Dhumal

Felicitation by Chandrakant Dada Patil (Revenue Minister of Maharashtra and member of the Maharashtra Legislative Council) of Sayli Dhumal for her achievement of First Rank in Certificate Course in Molecular Diagnostics (2021-22) during the first convocation of Skill Development Center of Punyashlok Ahilyadevi Holkar Solapur University, Solapur on 29.08.2022.

The Certificate Course is run by Department of Biotechnology, V.G. Shivdare College of Arts, Commerce and Science, Solapur is affiliated to Skill Development Center of the University.

Collaborations / MoUs

• ·         Memorandum of Understanding was signed between V.G. Shivdare College of Arts, Commerce and Science, Solapur and Nagappa Kadadi Memorial Laboratory, Solapur

• ·         Memorandum of Understanding was signed between V.G. Shivdare College of Arts, Commerce and Science, Solapur and Dr. Potdar Laboratories, Solapur

• ·         Linkage was active between V.G. Shivdare College of Arts, Commerce and Science, Solapur and Pradhan Laboratories, Solapur

• ·         Linkage was active between V.G. Shivdare College of Arts, Commerce and Science, Solapur and Ashwini Sahakari Rignalaya and Research Centre, Solapur

• ·         Linkage was active between V.G. Shivdare College of Arts, Commerce and Science, Solapur and Virus Research and Development lab. of Department of Microbiology of Dr. Vaisampayan Memorial Government Medical College, Solapur

• ·         Linkage was active between V.G. Shivdare College of Arts, Commerce and Science, Solapur and Dr. Kotnis Memorial Central Railway Hospital, Solapur

Result:

·         Batch 2021-22 – 100% pass